First Report of green Synthesis of Copper Oxide Nanoparticles from Brassica Tournefortii Leaves Extract and Their Antibacterial Activity
DOI:
https://doi.org/10.54361/LJMR.16.2B.06Keywords:
Antibacterial activity, Bioreduction method, Brassica tournefortii leaves extract, CuO nanoparticlesAbstract
In the present study, copper oxide nanoparticles (CuO NPs) were synthesized using a eco-friendly technique and evaluated their antibacterial activity. At the first time, Brassica tournefortii leaves extract mediated copper oxide-based nanoparticles which prepared by a bio-reduction method using an aqueous Brassica tournefortii leaves extract as both a reducing and stabilizing agent. Copper oxide nanoparticles were characterized by using ultraviolet-visible spectroscopy (UV-VIS), Fourier transform-infrared spectroscopy (FT-IR), x-ray diffraction (XRD) and scanning electron microscope (SEM) techniques. The synthesized CuO nanoparticles were also evaluated for their antibacterial activity against gram-positive (Staphylococcus aureus and Streptococcus spp) and gram-negative (Escherichia coli and Pseudomonas aeruginosa) bacterial strains. UV-Vis and FT-IR results showed that the successful formation and stabilization of copper oxide nanoparticles due to biomolecules in Brassica tournefortii extract. XRD results indicated that the CuO nanoparticles were monoclinic of size 27.73 nm. SEM image showed that CuO nanoparticles had spherical morphology. Furthermore, Antibacterial activity showed that CuO nanoparticles have good antibacterial agent against both gram positive and gram-negative organisms. The antibacterial assay revealed that Pseudomonas aeruginosa a maximum zone of inhibition (44 mm) at 30 mg/ml concentration of CuO nanoparticles.
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