Determination of Fluoride Concentration in Rosemary (Rosmarinus officinalis) using Selectiv- electrod technique

Abdulhakim B. Naffati; Mahmoud B. Agena

doi:10.54361/ljmr.17-08

Authors

Abdulhakim B. Naffati Libyan Medical Research Center Author
Mahmoud B. Agena Libyan Medical Research Center Author

DOI:

https://doi.org/10.54361/ljmr.17-08

Keywords:

Rosemary, fluoride, fluoride-selective electrode

Abstract

Background: Fluoride is characterized by high toxicity, especially in prolonged exposures. Some plants including Rosemary can higher of accumulate up to 20 000 times this element concentration in the environment. Consumption of highly fluoridated rosemary can contribute fluorosis and other body dysfunctions. Aims: This study aims to evaluate and optimize the extraction procedure of fluorides from the dried leaves of the plant, and compare the fluoride levels in collected samples. Methodology: nine of Rosemary samples from different countries including Libya, southern Serbia, Vojvodina and Istria were investigated in this study. Fluoride levels was determined potentiometrically using the calibration curve, via fluoride-selective electrode method based on lanthanum fluoride monocrystal. The sensitivity of the method was defined on the basis of 3SD criteria 3 using excel software. Results: fluoride concentration in investigated samples was varied. Libyan samples contained (1.8ppm) which is higher than other samples that ranged between 0.07 to0.49 ppm. However, all samples found to have fluoride concentration ranged with the recommended levels and less than the toxic or lethal doses. Conclusion: The modified potentiometric method using fluoride-selective electrode techniques for fluoride determination is highly effective with detection limit of 10 -9 mg/100g and is recommended in quantification of fluoride in Rosemary and other plant or spice samples. However, Results obtained by this study indicated that all samples contained different levels of in which all do not exceed the daily intake. Recommendations. Rosemary may offer an essential levels of required fluoride for daily intake. Further studies are recommended especially in areas with high fluoride content either in soil or via air pollution especially where rosemary is highly consumed to avoid fluorosis.

References

Choubisa, S. L. (2022). Radiological Findings More Important and Reliable in the Diagnosis of Skeletal Fluorosis. Austin Med Sci, 7(2), 1069.

Das TK, Susheela AK, Gupta IP, Dasarathy S, Tandon RK (1994) Toxic effects of chronic fluoride ingestion on the upper gastrointestinal tract. J Clin Gastroenterol 18:194–199. J Basic Med Sci 20:841–848.

de Oliveira FA, MacVinish LJ, Amin S, et al. (2017) The effect of fluoride on the structure, function, and proteome of intestinal epithelia. Environ Toxicol 33:63–71. 10.1002/tox.22495

DenBesten, P., & Li, W. (2011). Chronic fluoride toxicity: dental fluorosis. Fluoride and the oral environment, 22, 81-96.

Dyachenko, A.N. et al. (2016) ‘Physics and chemistry of the hydrogen fluoride production process from fluorine containing waste’, IOP Conference Series: Materials Science and Engineering, 135, p. 012012.

Follin-Arbelet B, Moum B (2016) Fluoride: a risk factor for inflammatory bowel disease? Scand J Gastroenter 51:1019–1024.

Godel, J.C. (2002). The use of fluoride in infants and children, Paediatrics & Child Health, Volume 7, Issue 8, October 2002, Pages 569–572,

Johnston, N. R., & Strobel, S. A. (2020). Principles of fluoride toxicity and the cellular response: a review. Archives of toxicology, 94(4), 1051–1069.

Klemm, R.B. and Klemm, M.E. (1970) ‘Adaptation of the AOAC fluorine method for determining fluoride in fish protein concentrate’, Journal of AOAC INTERNATIONAL, 53(1), pp. 3–6.

López, D. A., Franco, Ángela M., Estrada, J. J., & Zapata, J. A. (2009). Fluoride content in beverages of frequent intake by children at risk age of dental fluorosis. Medellín, 2006. Revista Facultad De Odontología Universidad De Antioquia, 19(2), 54–59.

Lubojanski, A., Piesiak-Panczyszyn, D., Zakrzewski, W., Dobrzynski, W., Szymonowicz, M., Rybak, Z., Mielan, B., Wiglusz, R. J., Watras, A., & Dobrzynski, M. (2023). The Safety of Fluoride Compounds and Their Effect on the Human Body-A Narrative Review. Materials (Basel, Switzerland), 16(3), 1242.

Luo, Jinlei, Dejiang Ni, Chunlei Li, Yaru Du, and Yuqiong Chen. "The relationship between fluoride accumulation in tea plant and changes in leaf cell wall structure and composition under different fluoride conditions." Environmental Pollution 270 (2021): 116283.

McDonagh, M. S., Whiting, P. F., Wilson, P. M., Sutton, A. J., Chestnutt, I., Cooper, J., ... & Kleijnen, J. (2000). Systematic review of water fluoridation. Bmj, 321(7265), 855-859.

Melo CGS, Perles JVCM, Zanoni JN, et al. (2017) Enteric innervation combined with proteomics for the evaluation of the effects of chronic fluoride exposure on the duodenum of rats. Nat Sci Rep 7

Mendes, A.L. et al. (2020) ‘A sample preparation method for fluoride detection by potentiometry with ion-selective electrode in medicinal plants’, Journal of Fluorine Chemistry, 231, p. 109459.

Miya, Kamal Shaiyad, and Vinay Kumar Jha. "Determination of Fluoride in Various Samples Using a Fluoride Selective Electrode." Journal of Analytical Sciences, Methods and Instrumentation 10.4 (2020): 97-103.

Nic M, Jirat J, Kosata B, eds. “IUPAC Compendium of Chemical Terminology” (Online edition, 2006)

Nigus, K. and Chandravanshi, B. S. (2016). Levels of fluoride in widely used traditional Ethiopian spices. Fluoride 49:165-177

Pandey, P.C. et al. (2014) ‘Fluoride-induced impact of aluminium industrial power plant on plants and human inhabiting areas’, Geofizika, 31(2), pp. 151– 168.

Sheridan RL, Ryan CM, Quinby WC Jr, et al. (1995) Emergency management of major hydrofluoric acid exposures. Burns 21:62–64.

Stefanaki, A. and van Andel, T. (2021) ‘Mediterranean aromatic herbs and their culinary use’, Aromatic Herbs in Food, pp. 93– 121.

Truong, X.V. (2020). Determining the content of toxic elements (Pb, Cd, and As) in herbal plants collected from different sites in northern Vietnam. Journal of Vietnamese Environment, 12, 70-77.

Ullah, R., Zafar, M. S., & Shahani, N. (2017). Potential fluoride toxicity from oral medicaments: A review. Iranian journal of basic medical sciences, 20(8), 841–848.

Wang, X. et al. (2022) ‘Biphasic functions of sodium fluoride (NAF) in soft and in hard periodontal tissues’, International Journal of Molecular Sciences, 23(2), p. 962.

Wong, M.H., Fung, K.F. and Carr, H.P. (2003) ‘Aluminium and fluoride contents of tea, with emphasis on brick tea and their health implications’, Toxicology Letters, 137(1–2), pp. 111–120.