AN IN-VITRO STUDY OF EFFECTS OF CENTELLA ASIATICA ON  PROLIFERATION RATE OF HUMAN PERIODONTAL LIGAMENT  FIBROBLASTS

Awatef M. Younis; Hoaida K.  Shawi; Mahmmed I.  Abu-Hassan

doi:10.54361/

Authors

Awatef M. Younis faculty of medical technology Az Zawia university, Zawia ,Libya Author
Hoaida K. Shawi faculty of medical technology Az Zawia university, Tripoli ,Libya Author
Mahmmed I. Abu-Hassan Faculty of Dentistry, University Teknologi MARA, Shah Alam, Malaysia Author

DOI:

https://doi.org/10.54361/

Keywords:

centella asiatica, human periodontal ligament fibroblast, proliferation

Abstract

Centella asiatica is a member of a tropical plant family which is considered to be a good source of phenolic compounds. The present study was designed to assess the effect of different concentrations of aqueous extract of Centella asiatica on the proliferation of human periodontal ligament fibroblast in-vitro. Aqueous extract of Centella asiatica was prepared and concentrations of 2-40 µg/ml were applied to the human periodontal ligament fibroblasts culture. The viability of human periodontal ligament fibroblast was investigated by MTT cell proliferation assay. The data were subjected to statistical analysis using ANOVA and Tukey's post hoc test. The concentration of aqueous extract of Centella asiatica that enhances highest proliferation of human periodontal ligament fibroblast cell was 20 µg/ml. the regeneration and protective effects of Centella asiatica can be utilize to treat the periodontal disease.

References

Melcher, A. H. (1970). Repair of wounds in the periodontium of the rat. Influence of periodontal ligament on osteogenesis. Arch Oral Biol 15:1183–1204.

Carnes, D. L., Maeder, C. L. and Graves, D.T. (1997). Cells with osteoblastic phenotypes can be explanted from human gingiva and periodontal ligament. J Periodontol 68:701–707.

Kotaro, Y., Katsujiro, S., Noriyuk, A., Masakazu, K., Keita, I., Hirotaka, S., Eto, H., Harunosuke, K., Toshitsugu, H. and Kiyonori, H. (2008). Cell-assisted lipotransfer for facial lipoatrophy: efficacy of clinical use of adipose-derived stem cells Dermatologic surgery: Official publication for American Society for Dermatologic Surgery. 34(9):1178-85.

Berkovitz, B. K. B. and Shore, R. C. (1995). Cells of the periodontal ligament. In The periodontal ligament in health and disease, 2nd edn (ed. B.K.B. Berkovitz, B. J. Moxham and H. J. Newman), pp. 9-33. London: Mosby-Wolfe.

Beertsen, W., Everts, V. and Vanden, H. A.(1974). Fine structure of fibroblasts in the periodontal ligament of the rat incisor and their possible role in tooth eruption. Arch Oral Biol 19: 1087-98.

Lekic, P., and McCulloch, C. A. G. (1998). Periodontal ligament cell populations: The central role of fibroblasts in creating a unique tissue. Arch Oral Biol. 245(2):327-341.

Pietruska, M. D. (2001). A comparative study on the use of Bio-Oss and enamel matrix derivative (Emdogain) in the treatment of periodontal bone defects. Eur J Oral Sci. 109:178-81.

Sculean, A., Chiantella, G. C., Windisch, P., Arweiler, N. B., Brecx, M. and Gera, I. (2005). Healing of intra-bony defects following treatment with a composite bovine- derived xenograft (Bio-Oss Collagen) in combination with a collagen membrane (Bio- Gide PERIO). J Clin Periodontal. 32:720-4.

Abraham, D., Lupoli, S., McWhirter, A., Plater-Zyberk, C., Piela, T.H. and Korn, J.H. (1991). Expression and function of surface antigens on scleroderma fibroblasts. Arthrit Rheum, 34:1164-1172.

Fries, K. M., Blieden, T., Looney, R. J., Sempowski, G. D., Silvera, M. R. and Willis, R. A. (1994). Evidence of fibroblast heterogeneity and the role of fibroblast subpopulations in fibrosis. Clin Immunol Immunopathol, 72:283-292.

Atherthon, A. J., O'Hare, M. J., Buluwela, L., Titley, J., Monaghan, P. and Paterson, H.F. (1994). Ectoenzyme regulation by phenotypically distinct fibroblast sub- populations isolated from the human mammary gland. J Cell Sci 107:2931-2939.

Hou, L. T. and Yaeger, J. A. (1993). Cloning and characterization of human gingival and periodontal ligament fibroblasts. J Periodontol 64:1209-1218.

Dennison, D. K., Vallone, D. R., Pinero, G. J., Rittman, B. and Caffesse, R. G. (1994). Differential effect of TGF-1l and PDGF on proliferation of periodontal ligament cells and gingival fibroblasts. J Periodontol. 65:641-648.

Ogata, Y., Niisato, N., Sakurai, T., Furuyama, S. and Sugiya, H. (1995). Comparison of the characteristics of human gingival fibroblasts and periodontal ligament cells. J Periodontol. 66:1025–1031.

Giannopoulou, C. and Cimasoni, G. (1996). Functional characteristics of gingival and periodontal ligament fibroblasts. J Dent. Res. 75:895-902. 16. Hassell, T. M. and Stanek, E. J. (1983). Evidence that healthy human gingiva contains functionally heterogeneous fibroblast subpopulations. Arch Oral Biol 28:617-625.

McCulloch, C. A. G. and Bordin, S. (1991). Role of fibroblast subpopulations in periodontal physiology and pathology. J. Periodontal Res. 26, 144-154.

Hakkinen, L. and Larjava, H. (1992). Characterization of fibroblast clones from periodontal granulation tissue in vitro. J Dent Res 71:1901-1907.

Irwin, C. R, Picardo, M., Ellis, I., Sloan, P., Grey, A. M. and McGurk, M. (1994). Inter- and intra-site heterogeneity in the expression of fetal-like phenotypic characteristics by gingival fibroblasts: potential significance for wound healing. J Cell Sci 107:1333 -1346.

Bou-Gharios, G., Osman, J., Black, C. and Olsen, I. (1994). Excess matrix accumulation in scleroderma is caused by differential regulation of stromelysin and TIMP-1 synthesis. Clin Chim Acta 231:69-78.

Stephens, P., Davies, K. J. and Al-Khateeb, T. (1996). Acomparison of the ability of intra-oral and extra-oral fibroblasts to stimulate extracellular matrix reorganization in a model of wound contration. J Dent Res.75; 1358-64.

Tajima, S. and Pimell, S. R. (1981). Collagen synthesis by human skin fibroblasts in culture: studies of fibroblasts explanted from papillary and reticular dermis. J Invest Dermatol.77; 410-2.

Schneider, E. L., Mitsui, Y. and Au, K. S. (1977). Tissue-specific differences in cultured human diploid fibroblasts. Exp cell Res.108:1-6.

De Lucia, C., Sertie, J. A. A., Camargo, E. A. and Panizza, S. (1997). Pharmacological and toxicological studies on Centella asiatica extract. Fitoterapia. 68: 413-416.

Sing, B. and Rastogi, R. P. (1969). Reinvestigation of the Triterpenes of Centella asiatica. Phytochemistry. 8: 917-921.

Brinkhaus, B., Lindner, M., Schuppan, D. and Hahn, E. G. (2000). Chemical, harmacological and clinical profile of the East Asian medical plant Centella asiatica: Review Article. Phytomedicine. 7(5): 427-448.

Sharma, R., Jaiswal, A. N., Kumar, S., Chaturvedi, C. and Tiwari, P. V. (1985). Role of brahmi in educable mentally retarded children. J. Res. Educ. Indian Med. No. (1-6), 55-77.

Karting, T. (1988). Clinical application of Centella asiatica (L) Urb. In herbs, spices and medicinal plants (Craker, L.E., and Simon, J.E. Eds.) Oxyx press, phoenix. 145- 173.

Maquart, F. X., Chastag, F., Simeon, A., Birembaut, P., Gillery, P. and Wegrowski, Y. (1999). Triterpenes from Centella asiatica stimulates extracellular matrix accumulation in rat experimental wounds. Connective Tissue Research .9:289-96.

Inamdar, P. K., Yeole, R. D., Ghogare, A. B. and De Souza, N. J. (1996). Determination of biologically active constituents in Centella asiatica. J. of Chromatography A. 742: 127- 130.

Bonte, F., Dumas, M., Chaudagn, C. and Meybeck, A. (1995). Asiaticoside and madecassoside comparative activities on human fibroblast typeI and III collagen secretion. Ann. Pharm. Fr., 53, 38-42.

Coldren, C. D., Hashim, P., Ali, J. M., Oh, S. K., Sinskey, A. J. and Rha, C. (2003). Gene expression changes in the human fibroblast induced by centella asiatica triterpenoids. Planta med. 69: 75- 732.

Lee, J., Jung, E. and Kim, Y. (2006). Asiaticoside induces human collagen I synthesis through TGFbeta receptor I kinase (T beta RI kinase) - independent smad signaling. Planta med. 72(4):324-328.

Mosmann, T. (1983). Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. Journal of Immunological Methods, 65, pp. 55–63.

Hussain, R. F., Nouri, A. M. E. and Oliver, R. T. D. (1993). A new approach for measurement of cytotoxicity using colorimetric assay. Journal of Immunological Methods. 160, pp. 89–96.

Lee, H. G. and Eun, H. C. (1999). Differences between fibroblasts cultured from oral mucosa and normal skin: implication to wound healing. Journal of dermatological science 21: 176-182.

DeBiasi, R. L., Squier, M. K. T., Pike, B., Wynes, M., Dermody, T. S. and Tyler, K. L. (1998). Reovirus-induced apoptosis is preceded by increased cellular calpain activity and is blocked by calpain inhibitors. J. Virol. 73:695-701.

Raballo, R., Rhee, J., Lyn-Cook, R., Leckman, J. F., Schwartz, M. L. and Vaccarino, F. M. (2000). Basic fibroblast growth factor (Fgf2) is necessary for cell proliferation, neurogenesis in the developing cerebral cortex. J Neurosci 20:5012-5023.

Cavanagh, J. F. R., Mione, M. C., Pappas, I. S. and Parnavelas, J. G. (1997). Basic Fibroblast Growth Factor Prolongs the Proliferation of Rat Cortical Progenitor Cells In Vitro Without Altering Their Cell Cycle Parameters. Cerebal Cortex.7: 293-302; 1047-3211.

Shimabukuro, Y., Terashima ,Y., Terashima, H., Ozasa, M., Terakura, M., Ikezawa, K., Hashikawa, T., Takedachi, M., Oohara, H., Yamada, S. and Murakami. (2008). Fibroblast growth factor-2 regulates expression of osteopontin in periodontal ligament cells. SJ Cell Physiol. 216(3):640-50.

Terashima ,Y., Shimabukuro, Y., Terashima, H., Ozasa, M., Terakura, M., Ikezawa, K., Hashikawa, T., Takedachi, M., Oohara, H., Yamada, S. and Murakami. (2008). Fibroblast growth factor-2 regulates expression of osteopontin in periodontal ligament cells. J Cell Physiol. 216(3):640-50.

Emecena, P., Akmanb, A. C., Hakkic, S. S., Hakkid, E. E., Demiralpb, B., T z mb, T. F. and Nohutcu, R. M. (2009). ABM/P-15 modulates proliferation and mRN synthesis of growth factors of periodontal ligament cells. Acta Odontologica Scandinavica, 67, 2, 65 – 73.

Denhardt, D. T, Giachelli, C. M. and Rittling, S. R. (2001). Role of osteopontin in cellular signaling and toxicant injury. Annu Rev Pharmacol Toxicol 41:723–749, 10.1146/annurev.pharmtox.41.1.723.

Standal, T., Hjorth-Hansen, H., Rasmussen, T., Dahl, I. M., Lenhoff, S., Brenne, A. T., Seidel, C., Baykov, V., Waage, A., Borset, M., Sundan, A. and Hjertner, O. (2004). Osteopontin is an adhesive factor for myeloma cells and is found in increased levels in plasma from patients with multiple myeloma. Haematologica, 89, 2, 174-182.