Isolation and identification of Rhodococcus and their effi-cacy in bioremediation of heavy metals in biomedical waste ash

Hanan S. Wees; Marwa A. Hasan; Huda S. Alrawiq

doi:10.54361/ljmr.16202

Authors

Hanan S. Wees Department of Biotechnology, Sabha University, Libya Author
Marwa A. Hasan Department of Zoology, Assistant professor, Sabha University, Libya Author
Huda S. Alrawiq Department of Botany, Assistant professor, Sabha University, Libya Corresponding Author

DOI:

https://doi.org/10.54361/ljmr.16202

Keywords:

Biomedical waste ash, Cadmium, Heavy metals, Rhodococcus, Temperature

Abstract

Biomedical waste (BMW) ash from BMW incineration remains poses a serious threat to the ecosystem because it contains large amounts of toxic heavy metals, which are often disposed of in regular landfills or directly into the soil and can contaminate soil and groundwater. This study aimed to develop a bioremediation technique to re-move the toxicity of ash before disposing of it in landfills. Bacteria belonging to the Rhodococcus genus were used in this study; one species was isolated from soil contaminated with livestock remains and another species was isolated from an agricultural area in Sabha city. The degradation ability of the bacteria was tested by meas-uring the concentration of the heavy metals by Atomic Absorption Spectroscopy (AAS) at different ranges of temperatures (25,30,35°C) and exposure times (2,4,6 and 8 days). The bacteria proved their ability to degrade 85% of Cadmium. The best removal rate of Cadmium was at 35 ° C on the eighth day. Our findings revealed that the Rhodococcus genus bacteria have high efficiency in removing Cadmium from samples of BMW ash and could be used as a potential agent to treat heavy metals contamination efficiently and reduce the negative impact of BMW ash on the environment.

References

M. M. Abd El-Salam, "Hospital waste management in El-Beheira governorate, Egypt," Journal of environmental manage-ment, vol. 91, no. 3, pp. 618-629, 2010. DOI: https://doi.org/10.1016/j.jenvman.2009.08.012

M. Sawalem, E. Selic, and J.-D. Her-bell, "Hospital waste management in Libya: A case study," Waste Management, vol. 29, no. 4, pp. 1370-1375, 2009. DOI: https://doi.org/10.1016/j.wasman.2008.08.028

B. Heera, "treatment and metal char-acterization of biomedical waste ash, J," Waste Manage, no. 2014. DOI: https://doi.org/10.1155/2014/956316

C. Li, K. Zhou, W. Qin, C. Tian, M. Qi, X. Yan, and W. Han, "A review on heavy metals contamination in soil: effects, sources, and remediation techniques," Soil and Sedi-ment Contamination: An International Jour-nal, vol. 28, no. 4, pp. 380-394, 2019. DOI: https://doi.org/10.1080/15320383.2019.1592108

J. Anitha and I. A. Jayraaj, "Isolation and identification of bacteria in biomedical wastes (BMW)," International journal of pharmacy and pharmaceutical sciences, vol. 4, no. 5, pp. 386-388, 2012.

B. Whitman, M. Goodfellow, P. Kämpfer, HJ. Busse, E. Trujillo , K-I. Suzuki and W. Ludwig,., Bergey's manual® of sys-tematic bacteriology: Volume five the actino-bacteria, part a. Springer,. 53(6): pp. 301-121.2012. DOI: https://doi.org/10.1007/978-0-387-68233-4

S. Aghaei, N. Bahador, and M. Bas-erisalehi, "Isolation and identification of bio-active compound producing Rhodococcus spp. isolated from soil samples," Internation-al Journal of Molecular and Clinical Microbi-ology, vol. 5, no. 1, pp. 463-468, 2015.

J. G. Cappuccino and C. T. Welsh, Mi-crobiology: a laboratory manual. Pearson Education, 11th pp.321-520. 2017.

R. Gorman and C. Adley, "An evalua-tion of five preservation techniques and con-ventional freezing temperatures of− 20° C and− 81° C for long‐term preservation of Campylobacter jejuni," Letters in applied mi-crobiology, vol. 38, no. 4, pp. 306-310, 2004. DOI: https://doi.org/10.1111/j.1472-765X.2004.01490.x

U. Epa, "Ecological effects test guide-lines: seed germination/root elongation tox-icity test," 1996.

S. Al-Sahlany, "Isolation and Identifi-cation of Bacillus cereus Bacteria and Using in Polyhydroxybutyrate Production," Uni-versity of Barsah, 2015.

Y.-Q. Zhang et al., "Rhodococcus yunnanensis sp. nov., a mesophilic actino-bacterium isolated from forest soil," Interna-tional Journal of Systematic and Evolution-ary Microbiology, vol. 55, no. 3, pp. 1133-1137, 2005. DOI: https://doi.org/10.1099/ijs.0.63390-0

D. P. Samarth, C. J. Chandekar, and R. K. Bhadekar, "Biosorption of heavy metals from aqueous solution using Bacillus licheni-formis," International Journal of Pure and Applied Sciences and Technology, vol. 10, no. 2, p. 12, 2012. DOI: https://doi.org/10.6088/ijes.00202030020

M. Rostom, K. A. Selim, A. Diab, G. Safwat, and N. A. Ibrahim, "Biological Strat-egy for Heavy Metal Removal from Industri-al Wastewater, "Elixir International Journal ". vol. 130, pp, 53161-5316 .2019.