Assessment of genetic variation and chemical composition of Origanumspp

H M Abushhewa; A T Abukreba; M S Khalifa; C  Lotti; L Ricciardi; V Bracuto; G Brunetti; L Verdini; A A Nass; W R Mohsen; M O B Gumma; G Mastro

doi:10.54361/ljmr.v14i1.01

Authors

H M Abushhewa Department of Biochemistry and Molecular Biology, Faculty of Medicine, University of Azzaytuna, Libya ;Department of Environmental Science and Chemistry, University of Bari “Aldo Moro”, Bari, Italy; Department of Biology, Unit of Genetics, University of Bari “Aldo Moro” Bari, Italy Author
A T Abukreba Biotechnology Research Centre, Tripoli, Libya Author
M S Khalifa Biotechnology Research Centre, Tripoli, Libya Author
C Lotti Department of Biology, Unit of Genetics, University of Bari “Aldo Moro” Bari, Italy Author
L Ricciardi Department of Biology, Unit of Genetics, University of Bari “Aldo Moro” Bari, Italy Author
V Bracuto Department of Biology, Unit of Genetics, University of Bari “Aldo Moro” Bari, Italy Author
G Brunetti Centre for Environmental Risk Assessment and Remediation, University of South Australia, Australia Author
L Verdini Department of Environmental Science and Chemistry, University of Bari “Aldo Moro”, Bari, Italy Author
A A Nass Biotechnology Research Centre, Tripoli, Libya Author
W R Mohsen Department of Pharmacology and Toxicology, Faculty of Medicine, University of Azzaytuna, Libya Author
M O B Gumma Department of Anatomy, Faculty of Medicine, University of Azzaytuna, Libya Author
G Mastro Department of Environmental Science and Chemistry, University of Bari “Aldo Moro”, Bari, Italy Author

DOI:

https://doi.org/10.54361/ljmr.v14i1.01

Keywords:

Origanumspp, Essential oil, Gas chromatography–mass spectrometry, Random Amplification of Polymorphic DNA, Amplified fragment length polymorphism

Abstract

The study was carried out on 23 entries of Origanumcollected from different areas of south Italy. The 23 entries were characterizedvia determining the chemical composition of their essential oils and genetic variability. The gas-chromatography of the essential oils of oregano accessions allowed the detection of 44 components with the predominance of carvacrol, thymol, linalyl acetate, γ-terpinene, o-cimene, s-caryophylleneand cis-ocimene. A high variability in the main components concentration was revealed except in the case of the accessions 13, 14 and 15 where the linalyl acetate ranging between 51.27 and 60.93%, outlining a new oregano chemotype.Using hierarchical cluster analysis, four main groups of samples were observed. Genetic variability using the RAPD analysis was not able to reveal clear polymorphism PCR patterns useful to distinguish the entries.So that, we decided to conduct further molecular analyses to determine the genetic variation among the entries under investigation, using AFLPs a powerful tool to perform phylogenetic analysis. This technique shows a high capability in detecting genetic variation. Combination between fluorescent system and polyacrylamide gels allows obtaining large number of bands (225 to 557). Finally, thecurrent study shows thatthe Dendrogram of genetic similarity of Origanum is widely variable amongst genotypes of this plant.

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