Investigation of the effect of POMC peptides on dermal fibroblast migration and its role in wound healing
DOI:
https://doi.org/10.54361/Keywords:
Fibroblasts, immunohistochemistry, wound healing, POMIC peptidesAbstract
he human skin contains several different fibroblast subtypes including dermal sheath (DS), dermal papilla (DP) and inter-follicular dermis cells (DF). It has been shown that quicker healing and less scar formation is related toproportionally fewer numbers of DS and DP fibroblasts. TGF-β plays a crucial role in wound healingby attracting fibroblasts and promoting their synthesis of collagens I, III, and V, proteoglycans, fibronectin and other extra cellular matrix (ECM) components; and that the POMC peptides α-MSH/ ACTH may ameliorate hypertrophic scaring by antagonizing TGF-β. The aim of this project was to assess the role of POMC peptides α-MSH/ ACTH in modulating migratory activity of fibroblasts in vitro using scratch assay with or without POMC peptides and the transwell assay in the presence of the extracellular protein fibronectin. Transwell assay showed that fibronectin increased fibroblast sub-types migration with DS cells migrated through fibronectintranswells more effectively than other fibroblast sub-types. POMC peptides, ACTH induced the greatest increase in migration with fibronectin coated wells. In addition, scratch assay showed that the fibroblast migration was significant in the presence of the POMC peptides.
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Copyright (c) 2017 Samia Tayeb Hawisa, Hana Fahelbum, Salah Bahroun, Naziha Mansuri (Author)
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