THE EFFECT OF MEDICINAL PLANTS AND THE GENTAMICIN   AGAINST SELECTED BACTERIA : IN VITRO STUDY

Fathi Abdallah Shakurfow; Ali Salem Faraj  Edalim; Osama Milad  Elgutt; Abdelkrem Mohamed Bashir  Abdalla; Abd urraouf  Zaet

doi:10.54361/ljmr15.1.12

Authors

Fathi Abdallah Shakurfow Medical Laboratory Department, Faculty of Medical Technology, El-Mergib Uni, Libya Author
Ali Salem Faraj Edalim Medical Laboratory Department, Faculty of Medical Technology, El-Mergib Uni, Libya Author
Osama Milad Elgutt Medical Laboratory Department, Faculty of Medical Technology, El-Mergib Uni, Libya Author
Abdelkrem Mohamed Bashir Abdalla Medical Laboratory Department, Faculty of Medical Technology, Zawia Uni, Libya Author
Abdurraouf Zaet Dental Technology Department, Faculty of Medical Technology, Zawia Uni. Libya Author

DOI:

https://doi.org/10.54361/ljmr15.1.12

Keywords:

garlic, ginger, acacia, thyme, Gentamicin

Abstract

Antimicrobial-resistant bacteria are becoming more serious danger to worldwide public health. As a result, the efficacy of traditional antimicrobials is fast falling, posing a threat to healthcare providers’ capacity to treat common infection. Bacterial infective diseases are an important reason of sickness and death globally. Several medicine resistances in human pathogenic microorganisms had develop because of undiscriminating custom of commercial anti-microbial medicines.The main objective of the present study is, to evaluate the ability of the plants extract, to inhibit the growth of pathogenic bacteria with and without antibiotics and non-antibiotics drugs. Also, to enhance the activity of antibiotics or non-antibiotics drugs against selective pathogenic bacteria, to investigate antibacterial effects of thyme crude extract (Tm), ginger crud extract (Gi), garlic crud extract (Ga) and acacia crud extract (AC) against Escherichia coli ( E.coli) and Staphylococcus aureus (S.aureus) and to investigate the ability of increasing the potency of Gentamycin antibiotic against E. coli and S. aureus by mixed with thyme crude extract (Tm), ginger crud extract (Gi), garlic crud extract (Ga) and acacia crud extract (AC). Material and methods, in this study used gentamicin mixed with for types of crud extracts garlic, ginger, acacia, and thyme against two types of bacteria E. coli and S. aureus. Used disc diffusion method. Results found that three types had increasing the effect of gentamicin against S. aureus and had not affect against E. coli. Also, increased the potency of gentamicin against S. aureus when combined with garlic, thyme and acacia crud extracts.

References

Cragg, G. M., and Newman, D. J. (2013); Natural products: A continuing source of novel drug leads. BiochBiophysActa 1830(6):3670-95.

Abdel Rahman, S., Abd-Ellatif, S., Deraz, S. and Khalil, A. (2011); Antibacterial activity of some wild medicinal plants collected from western Mediterranean coast, Egypt: Natural alternatives for infectious disease treatment. African Journal of Biotechnology, 10(52):10733-43. DOI: https://doi.org/10.5897/AJB11.007

Shakurfow, F. A. A., Buazzi, M. M., and Gamal, M. A., Assessment of antimicrobial activity of onion (Allium cepa) and garlic (Allium sativum) extracts on Listeria monocytogenes; in vitro study. Lebda Medical Journal. 1(1):1-5.

Harris, J. C., Plummer, S., Turner, M. P., Lloyd, D. (2000); The Microaerophilic Flagellate Giardia Intestinalis: Allium sativum (Garlic) is an Effective Antigiardial. Microbiology journal. 146 (12):3119-27. DOI: https://doi.org/10.1099/00221287-146-12-3119

Nakamoto, M., Kunimura, K., Suzuki, J., and Kodera, Y. (2020); Antimicrobial properties of hydrophobic compounds in garlic: Allicin, vinyldithiin, ajoene and diallylpolysulfides (Review). Spandidos publications. Experimental and therapeutic medicine. 19(2):1550-1553 DOI: https://doi.org/10.3892/etm.2019.8388

Sabulal, B., Dan, M., Kurup, R., Pradeep, N. S., Valsamma, R. K., and George, V. (2006); Caryophyllene-Rich Rhizome Oil of Zingibernimmoniifrom South India: Chemical Characterization and Antimicrobial Activity. Phytochem 67: 2469-2473. DOI: https://doi.org/10.1016/j.phytochem.2006.08.003

Kawai T., (1994); Anti-emetic principles of Magnolia obovataBark and ZingiberofficinaleRhizome. Planta Med. 60(1):17-20. DOI: https://doi.org/10.1055/s-2006-959399

Spicer, N., Barnes R., and Timberlake, J., (2007); Acacia handbook. DFID (2010). Short-term clinical effects of Forestry Research Programme, U.K.

Pradeep, A. R., Happy, D. and Garg, G. (2010); Short-Term Clinical Effects of Commercially Available Gel Containing Acacia Arabica: a randomized controlled clinical trial. Australian Dental Journal, 55:65-69. DOI: https://doi.org/10.1111/j.1834-7819.2009.01180.x

Omidbaigi, R. and Nejad, R. A.. (2000); The Influence Of Nitrogen Fertilizer and Harvested Time on The Productivity of T. vulgaris. Inter. J. Horti. Sci., 6(3): 43-46. DOI: https://doi.org/10.31421/IJHS/6/4/219

Jouda, M. M., Elbashiti, T., Masad, A., Albayoumi, M., (2015); The Antibacterial Effect of Some Medicinal Plant Extracts and Their Synergistic Effect With Antibiotics. Research Article 5(2):23-33.

Al-Muhna, B. M., Study of the Inhibitory Effect of Thymus vulgaris and Eucalyptus camaldulensis on In vitro Growth of Listeria monocytogenes. (2010); Kufa Journal For Veterinary Medical Sciences 1(1). DOI: https://doi.org/10.36326/kjvs/2010/v1i14240

Arshad, M. and Shadab, M., (2017); Zingiberofficinale Extract: Antimicrobial Properties Phytochemical Screening, Drug Likeness and Physicochemical Studies. Ejpmr. 4(3):364-368.

Wei, Q. Y., Ma, J. P., Cai, Y. J., Yang, L., and Liu Z. L., (2005); Cytotoxic and Apoptotic Activities of Diarylheptanoids and Gingerol-Related Compounds from The Rhizome of Chinese Ginger. Journal of ethnopharmacology. 102(2):177-184. DOI: https://doi.org/10.1016/j.jep.2005.05.043

Bellik, Y., (2014), Total antioxidant activity and antimicrobial potency of the essential oil and oleoresin of Zingiberofficinale Roscoe. Asian Pacific Journal of Tropical Disease. 4(1):40-44 DOI: https://doi.org/10.1016/S2222-1808(14)60311-X

Gupta, S., and Ravishankar, S. (2005); A Comparison of the Antimicrobial Activity of Garlic, Ginger, Carrot, And Turmeric Pastes Against E. coli O157:H7 in Laboratory Buffer And Ground Beef. Foodborne Pathog Dis. 2(4):330-40. DOI: https://doi.org/10.1089/fpd.2005.2.330

Karuppiah P. and Rajaram S. (2012) Antibacterial Effect of Allium sativum cloves and Zingiberofficinale Rhizome Against Multiple-Drug Resistance Clinical Pathogens. Asian J Biomedicine.; 2(8):597-601. DOI: https://doi.org/10.1016/S2221-1691(12)60104-X

Mohamedin, A., Elsayed A., and Shakurfow, F. A.Molecular Effects and Antibacterial Activities of Ginger Extracts Against Some Drug Resistant Pathogenic Bacteria (2018); Egypt. J. Bot., 58(1):133 -143.

Lawrence R., Jeyakumar E. and Gupta A. (2015); Antibacterial Activity of Acacia arabica (Bark) Extract Against Selected Multi Drug Resistant Pathogenic Bacteria Int. J. Curr. Microbiol. App. Sci. Special Issue-1: 213-222.

Park, M., Bae, J., Lee, D. S., (2008); Antibacterial Activity of [10]-Gingerol and [12]-Gingerol Isolated from Ginger Rhizome Against Periodontal Bacteria. Phytotherapyresearch : PTR 22(11):1446-1449. DOI: https://doi.org/10.1002/ptr.2473